How To Perform DNA Extraction Using Phenol Chloroform Method 🧑🏻‍🔬🧬👇
10:09
YouTubeBiotech Made Easy
How To Perform DNA Extraction Using Phenol Chloroform Method 🧑🏻‍🔬🧬👇
Genomic DNA (gDNA) extraction using phenol/chloroform utilize detergents such as sodium dodecyl sulfate (SDS) or cetyltrimethylammonium bromide (CTAB) for cell lysis. After cell lysis, phenol/chloroform/isoamyl alcohol (25:24:1) is added to separate lipids and other proteins into the organic phase, and the gDNA into the aqueous phase. After ...
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Get the most out of your critical samples with isolation of DNA up to 500kb. The Wizard® HMW DNA Extraction Kit is specifically designed to isolate high-molecular-weight (HMW) DNA that will provide strong performance in long-read sequencing. Works with a range of sample types, including whole blood, plant leaf, tissue culture cells and Gram-positive and Gram-negative bacteria. http://bit.ly/2T6ML26 | Promega Corporation
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